maxiscript sp6 vitro transcription kit Search Results


direct-zol rna microprep  (Zymo Research)
99
Zymo Research direct-zol rna microprep
Direct Zol Rna Microprep, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
direct-zol rna microprep - by Bioz Stars, 2026-10
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sp6 rna polymerase  (New England Biolabs)
96
New England Biolabs sp6 rna polymerase
Sp6 Rna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+vitro+transcription+kit/SP6+RNA+Polymerase/custom%40m0207%4019556782
Average 96 stars, based on 1 article reviews
sp6 rna polymerase - by Bioz Stars, 2026-10
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sp6 or t7 riboprobe synthesis kit  (Promega)
90
Promega sp6 or t7 riboprobe synthesis kit
Sp6 Or T7 Riboprobe Synthesis Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+vitro+transcription+kit/t3+or+t7+polymerase/pmc03664763-101-5-11
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sp6 or t7 riboprobe synthesis kit - by Bioz Stars, 2026-10
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pgemt  (Promega)
90
Promega pgemt
Pgemt, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pgemt - by Bioz Stars, 2026-10
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template dna  (Thermo Fisher)
99
Thermo Fisher template dna
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
Template Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+vitro+transcription+kit/DNA/pmc00121457-142-15-25
Average 99 stars, based on 1 article reviews
template dna - by Bioz Stars, 2026-10
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qiaquick pcr purification kit  (Qiagen)
99
Qiagen qiaquick pcr purification kit
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
Qiaquick Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+vitro+transcription+kit/QIAquick+PCR+Purification+Kit/pmc03076716-462-7-11
Average 99 stars, based on 1 article reviews
qiaquick pcr purification kit - by Bioz Stars, 2026-10
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hindiii  (New England Biolabs)
99
New England Biolabs hindiii
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
Hindiii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+vitro+transcription+kit/HindIII/custom%40r0104%4010%2E1038%2Fs41563-019-0368-6
Average 99 stars, based on 1 article reviews
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ecori  (New England Biolabs)
99
New England Biolabs ecori
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
Ecori, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+vitro+transcription+kit/EcoRI/custom%40r0101%4010%2E1038%2Fs41563-019-0368-6
Average 99 stars, based on 1 article reviews
ecori - by Bioz Stars, 2026-10
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pcs2 hspcas9  (Addgene inc)
95
Addgene inc pcs2 hspcas9
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
Pcs2 Hspcas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+vitro+transcription+kit/pCS2%2BhSpCas9+(Plasmid+%2351815)/pmc07452054-35-25-26
Average 95 stars, based on 1 article reviews
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dna purification kit  (Promega)
90
Promega dna purification kit
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+vitro+transcription+kit/wizard+dna+purification+kit/pmc02956009-93-39-42
Average 90 stars, based on 1 article reviews
dna purification kit - by Bioz Stars, 2026-10
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riboprobes  (Thermo Fisher)
99
Thermo Fisher riboprobes
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
Riboprobes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+vitro+transcription+kit/Fluorescein/pmc04509676-91-0--1
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32p]utp  (ICN Pharmaceuticals)
90
ICN Pharmaceuticals 32p]utp
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
32p]Utp, supplied by ICN Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+vitro+transcription+kit/g+32p+atp/10__1074_slash_jbc__m210526200-92-18-19
Average 90 stars, based on 1 article reviews
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Image Search Results


The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by an antisense DNA oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.

Journal:

Article Title: Sequence Divergence in the 3? Untranslated Regions of Human ?- and ?-Globin mRNAs Mediates a Difference in Their Stabilities and Contributes to Efficient ?-to-? Gene Developmental Switching

doi:

Figure Lengend Snippet: The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by an antisense DNA oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.

Article Snippet: Internally 32 P-labeled antisense-oriented RNA probes for RNase protection assays were transcribed in vitro from template DNA by using SP6 RNA polymerase (SP6 Maxiscript kit; Ambion).

Techniques: Transgenic Assay, Sequencing, Acrylamide Gel Assay, Hybridization, Labeling, Rnase H Assay